Journal: Food Science & Nutrition

Article Title: Dihydroquercetin Attenuates Silica‐Induced Pulmonary Fibrosis by Modulating the Gut Microbiota and the Serum Metabolites in Mice

doi: 10.1002/fsn3.71389

Figure Lengend Snippet: DHQ treatment attenuated silica‐induced pulmonary fibrosis in C57/BL6 mice. (A, B) DHQ treatment increased the body weight and decreased pulmonary index in silicosis model mice. The changes of body weight (C) The levels of pro‐inflammatory cytokines (IL‐1β, TNF‐α, and TGF‐β) in serum from different groups at day 21 were detected by ELISA assay. (D) Representative pictures (×200) of HE‐stained and Masson‐stained lung sections from mice on day 21 were shown. Bar = 100 μm. (E, F) The inflammation and fibrosis score numbers of 0–3, corresponding to the grades of –, +, ++, and +++, were evaluated by experienced pathologists in a blinded fashion. (G) Representative results of western blot for α‐SMA, collagen I and fibronectin in lung tissues and the quantification of results. Data are shown as mean ± SD. All experiments were repeated three times. # p < 0.05, ## p < 0.01 vs. the control group; * p < 0.05, ** p < 0.01 vs. the SiO 2 group.

Article Snippet: Sigma‐Aldrich provided the SiO 2 (Cat#S5631) particles (around 80% diameter 1‐5 μm), which were filtered through sedimentation following Stokes' law, underwent acidic hydrolysis, and were baked overnight at 200°C for 16 h. Interleukin‐1β (IL‐1β) (CSB‐E08054m), tumor necrosis factor‐α (TNF‐α) (CSB‐E04741m), and transforming growth factor‐β (TGF‐β) (CSB‐E04726m) were acquired from Cusabio Biotechnology in Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Control

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 improve inflammation and fibrosis in vitro . (A) Splenocytes from C57BL/6 mice (n = 4-6) were stimulated with each strain separately (at 0.1, 1, or 10 μg/mL) or vehicle (saline) for 2 h and then cultured with anti-CD3 Ab (2 μg/mL) or LPS (500 ng/mL) for 3 days. The levels of IFN-γ, IL-17 and IL-10 in the culture supernatant were determined via ELISA. (B) CCD-18Co cells were treated with each strain separately (at 10 μg/mL) or vehicle (saline) in the presence of TGF-β (10 ng/mL) for 24 h. The levels of fibronectin were assessed via immunoblotting. Values are presented as means ± SDs. Data are representative of two independent experiments.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: In Vitro, Saline, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 reduce colonic fibrosis in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Each strain was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). On day 11, colon tissue sections were stained with Masson’s trichrome and Abs against TGF-β, Col1, and α-SMA. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. *P < 0.05, ****P < 0.0001.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: Injection, Control, Staining

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: Postbiotics from L. curvatus DCF0620 reduce colonic fibrosis and inflammation in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Then, 200 μL of postbiotics (100 mg/mL in PBS) was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). (A) On day 12, colon tissue sections were stained with Abs against TGF-β, Col1, and α-SMA. (B) On day 12, the sections were stained with Abs against TNF-α, IL-1β, IL-6, and IL-17. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. ****P < 0.0001.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: Injection, Control, Staining